Researchers introduce method of coloring microscopic corallarvae to aid tracking for conservation and reef restoration plos PLOSBiology
used to induce settlement. The intensity of stain for each replicate was scored ordinally by a single observer , following the same protocol as the first experiment, and imaged using Toupview software on a dissecting microscope. White balance and black balance were set within the software prior to imaging. To test for differences in larval survival and larval settlement among treatments, we used a binomial GLM using the “glm” function in the stats package in R .
Larvae were stained with Nile blue using a concentration of 1,000 mg lfor 60 minutes to contrast the natural larval colors. Stained and natural larvae were visually assessed under light microscopy. Stained larvae were then released within a net enclosure containing preconditioned settlement tiles within Lizard Island lagoon to retain larvae during the transition from planktonic larvae to benthic settlement.
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