Nature research paper: Cryo-EM structure of a type IV secretion system
and 3.28 Å map. These maps were used for symmetry analysis of the OMCC O-layer and I-layer. b, workflow used to generate the O-layer C14 2.48 Å map and the I-layer C16 3.08 Å map. These maps were used to build and refine the atomic O- and I-layer models, respectively. c, workflow used to generate the initialmodel for the IMC-Arches-Stalk map. d, workflow used to generate the IMC-Arches-Stalk C1 6.
OMCC C1 map. The section is taken through the helical trans-membrane region of the O-layer. Middle Left: the non-averaged and unsharpened C1 map of the OMCC determined at a resolution of 3.28 Å colour-coded as in Fig.. The contour level is indicated. The three filled lines and two dashed arrows labelled 1 to 5 indicate where the map sections have been taken for analysis shown at right and at bottom .
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