Graphene grids enhance electron microscopy imaging with high uniformity, inertness, and mechanical strength, ideal for high-resolution biological samples.
By Rebecca Ingle, Ph.DMay 17 2024Reviewed by Lexie Corner Support grids are a key part of electron microscopy measurements; the choice of the grid can directly influence the quality and accuracy of the final image. This is particularly true for transmission electron microscopy , where samples must be sufficiently thin to allow for electron beam transmission and reduce unwanted scatter.
It is very challenging to avoid any contribution of the grid to the final electron microscopy image, especially as grids can contribute to electron scattering, which can ultimately diminish the effective resolution of the electron microscope. Additionally, depending on the elements involved, certain irregular grid materials can cause poor contrast between the substrate and grid.
Some graphene grids can even be used to protect samples.7 With the right functionalization, graphene grids can help improve particle distribution across the grid and, when combined with spacers, protect particles from surfaces and environments that may cause oxidation or damage. For some applications, it is sufficient to coat an electron microscopy grid made from another material, such as gold, with a graphene layer.9 It can be challenging to assess the quality of graphene grids, and for research groups that do not have specialists in graphene synthesis, it can also be challenging to make grids efficiently. These are some reasons behind the great demand for simplified synthesis methods.
Graphene Grid Properties Nanoparticle Imaging Transmission Electron Microscopy (TEM)
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