Nature research paper: Molecular basis for the initiation of DNA primer synthesis
. Conserved amino acids mutated in this study are shown in yellow. Non-conserved amino acids are shown in red. Conserved catalytic motifs I, II and III are in black rectangles as indicated. was added into the reaction containing 1 µM ssDNA mixed sequence template , 100 µM non-labelled dNTP mix and 10 µM FAM-γGTP . The reactions were incubated at 50 °C for 30 min. ‘C’ indicates a control reaction without protein. Oligonucleotide length markers are shown on the left of the gel.
Close-up view of Mn bound in the A site of the MsCAPP dGTP complex, showing octahedral coordination to dGTP, ethylene glycol and surrounding acidic residues with distances labelled in Å . Close-up view of Mn bound in the B site of MsCAPP dGTP complex, showing octahedral coordination to dGTP, DxD motif and a water molecule, with distances labelled in Å . Mn – purple spheres..
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