Astronomers create new microwave map of the MilkyWay and beyond RoyalAstroSoc
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Systems analysis of the effect of hydrogen sulfide on the growth of Methylococcus capsulatus Bath - Applied Microbiology and BiotechnologyAbstract Methanotrophs are bacteria capable on growing on methane as their sole carbon source. They may provide a promising route for upgrading natural gas into more valuable fuels and chemicals. However, natural gas may contain significant quantities of hydrogen sulfide. Little is known about how hydrogen sulfide affects the growth and physiology of methanotrophs aside from a few studies showing that it is inhibitory. This study investigated how hydrogen sulfide affects the growth and physiology of the model methanotroph, Methylococcus capsulatus Bath. Growth studies demonstrated that hydrogen sulfide inhibits the growth of M. capsulatus Bath when the concentration exceeds 0.5% (v/v). To better understand how hydrogen sulfide is inhibiting the growth of M. capsulatus Bath, transcription and metabolite concentrations were profiled using RNA sequencing and gas chromatography-mass spectrometry, respectively. Our analysis of the differentially expressed genes and changes in metabolite concentrations suggests that hydrogen sulfide inhibits cellular respiration. The cells respond to sulfide stress in part by increasing the rate of sulfide oxidation and by increasing the expression of sulfide quinone reductase and a putative persulfide dioxygenase. In addition, they reduce the expression of the native calcium-dependent methanol dehydrogenase and increase the expression of XoxF, a lanthanide-dependent methanol dehydrogenase. While the reason of this switch in unknown, XoxF has previously been shown to be induced by lanthanides or nitric oxide in methanotrophs. Collectively, these results further our understanding of how methanotrophs respond to sulfide stress and may aid in the engineering of strains resistant to hydrogen sulfide. Key points • Hydrogen sulfide inhibits growth of Methylococcus capsulatus Bath • Sulfide stress inhibits cellular respiration • Sulfide stress induces XoxF, a lanthanide-dependent methanol dehydrogenase
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M3GAN Has a Surprising Connection to The Polar Express, Reveals EPM3GAN Executive Producer reveals an unexpected connection to the Polar Express. “She looked like the real-life version of a ‘Polar Express’ character — like a CG character that shouldn’t actually exist.'
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Polar Lounge returns to Brooklyn hotel rooftop for a chill night out experience | amNewYorkFor the second year in a row, Harriet’s Rooftop at 1 Hotel Brooklyn Bridge is hosting its Polar Lounge installation. The lounge, located at 60 Furman Street,
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This 3-Week Expedition to the Arctic Circle Lets You See Polar Landscapes and Wildlife in LuxuryYou will explore the North’s vast and rugged terrain, from Greenland to Norway.
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In Norway, sled dogs train and play in warming ArcticBOLTERDALEN, Norway (AP) — Out of the dark, frozen vastness in this Arctic valley, a chorus of barks and howls trumpeted the return of Green Dog's oldest puppies from a dogsledding training run through the polar night on Tuesday afternoon.
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Lasers reveal massive, 650-square-mile Maya site hidden beneath Guatemalan rainforestWhile conducting an aerial survey of northern Guatemala, researchers detected a sprawling Maya site.
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