A Nature research paper investigates silent mutations in KRas and how they can be exploited therapeutically to inhibit oncogene signaling in cancer
, Morpholino oligo pre-treatment strategy. H650 cells were pre-treated with 10 μM control vivo-morpholino and target vivo-morpholino for 1 to 4 days as 3D suspension cells. After drug washout, cells were cultured in growth media and cell viability was evaluated on day 8experiments. After pre-treatment with 10 μM vivoMor-CTRL and target vivo-morpholino for 1 to 2 days as 3D suspension cells, drugs were washed out and cells were subcutaneously implanted into mice.
H650 cells were pre-treated with vivoMor-CTRL or vivoMor-4Extended Data Fig. 8 Intra-tumoral injection of vivo-morpholino in H650 xenograft models., Images of KRAS-specific PCR amplicons generated from the cDNA of H650 xenograft tumors that were treated with daily intra-tumoral injection of morpholino for 7days. Fraction of exon 3 skipping is defined as the band intensities of “skipped/” as measured by ImageJ . M: 100 bp-marker.
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